Acyl Lipids: enzymes

ARABIDOPSIS
ACYL-LIPID METABOLISM

ENZYME & PROTEIN DATA

Phosphatidate Phosphatase

H₂O

1,2-diacylglycerol-phosphate

1,2-diacylglycerol

Brenda: [3.1.3.4]
IUBMB: [3.1.3.4]
ExPASy: [3.1.3.4]
ExplorEnz: [3.1.3.4]
PDB: [3.1.3.4]

Enzyme Pathways

Prok Gal/Sulpho I | Euk Gal/Sulpho | TAG Synthesis | Euk Phospholipid | Signaling

Locus Data & Links (May need to scroll right or left to show columns)

Locus Id (TAIR Link)	Abbrev.	Mutant(s) (click for pop-up)	Pathway(s)	Subcelluar location	Evidence for function	Key References	Comments	ESTexpression in other oilseeds
At1g15080	LPP2	lpp2	Prok Gal/Sulpho I Euk Gal/Sulpho Signaling		Mutant Char.; Heterol Exp'n.	2 Refs Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308. Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117.	Heterologous protein exhibits both PA and DGPP phosphatase activity(Ref1), mutant accumulates PA during germination and is hypersensitive to ABA (Ref2)	Data
At2g01180	LPP1	lpp1	Prok Gal/Sulpho I Euk Gal/Sulpho Signaling	Plastid	Mutant Char.; Heterol Exp'n.	2 Refs Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308. Franca, M.G.C., Matos, A.R., D'Arcy-Lameta, A., Passaquet, C., Lichtle, C., Zuily-Fodil, Y., and Pham-Thi, A.T. (2008). Cloning and characterization of drought-stimulated phosphatidic acid phosphatase genes from Vigna unguiculata. Plant Physiology and Biochemistry 46 , 1093-1100.	Heterologous protein exhibits both PA and DGPP phosphatase activity	Data
At3g02600	PP; AtLPP3		Prok Gal/Sulpho I Euk Gal/Sulpho Mito LipoPolySach Signaling	Mitochondria	Seq. Similarity Seq. Similarity; Heterol Exp'n.	3 Refs Pierrugues, O., Brutesco, C., Oshiro, J., Gouy, M., Deveaux, Y., Carman, G.M., Thuriaux, P., and Kazmaier, M. (2001). Lipid phosphate phosphatases in Arabidopsis?Regulation of the AtLPP1 gene in response to stress. J. Biol. Chem. 276 , 20300-20308. Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117. Paradis, S., Laura Villasuso, A., Aguayo, S.S., Maldiney, R., Habricot, Y., Zalejski, C., Machado, E., Sotta, B., Miginiac, E., and Jeannette, E. (2011). Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. Plant Physiology and Biochemistry 49 , 357-362.		Data
At3g09560	PAH1	pah1	Euk Phospholipid	Endomembrane Cytosol	Mutant Char.; Heterol Exp'n.; Seq. Similarity; Comple. of yeast mutant	4 Refs Han, G.S., Wu, W.I., and Carman, G.M. (2006). The Saccharomyces cerevisiae lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J. Biol. Chem. 281 , 9210-9218. Nakamura, Y., Koizumi, R., Shui, G., Shimojima, M., Wenk, M.R., Ito, T., and Ohta, H. (2009). Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation. Proc. Natl. Acad. Sci. USA 106 , 20978-20983. Eastmond, P.J., Quettier, A.L., Kroon, J.T.M., Craddock, C., Adams, N., and Slabas, A.R. (2010). Phosphatidic acid phosphohydrolase 1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in Arabidopsis. Plant Cell 22 ,2796-2811. Mietkiewska, E., Siloto, R.M.P., Dewald, J., Shah, S., Brindley, D.N., and Weselake, R.J. (2011). Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1 Delta mutant strain of Saccharomyces cerevisiae. Febs Journal 278 , 764-775.	Seq. Similarity to yeast PAH1; Complements yeast PAP mutant; mutant characterization;pah1 pah2 double mutant has decreased soluble PAP activity and during phosphate starvation phospholipid remodelling to DGDG i decreased	Data
At3g18220	LPP4		Prok Gal/Sulpho I Euk Gal/Sulpho Signaling		Seq. Similarity	1 Ref Katagiri, T., Ishiyama, K., Kato, T., Tabata, S., Kobayashi, M., and Shinozaki, K. (2005). An important role of phosphatidic acid in ABA signaling during germination in Arabidopsis thaliana. Plant J. 43 , 107-117.		Data
At3g50920	LPP ε1	lppε1	Prok Gal/Sulpho I Euk Gal/Sulpho Signaling	Plastid	Mutant Char.; Heterol Exp'n.	1 Ref Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.	Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism	Data
At3g58490	LPP δ		Prok Gal/Sulpho I Euk Gal/Sulpho Sphingolipid I Signaling	Endomembrane (GFP)	Seq. Similarity	3 Refs Marion, J., Bach, L., Bellec, Y., Meyer, C., Gissot, L., and Faure, J.D. (2008). Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings. Plant Journal 56 , 169-179. Tsegaye, Y., Richardson, C.G., Bravo, J.E., Mulcahy, B.J., Lynch, D.V., Markham, J.E., Jaworski, J.G., Chen, M., Cahoon, E.B., and Dunn, T.M. (2007). Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J. Biol. Chem. 282 , 28195-28206. Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.	suggested function:LCB-P phosphatases (Ref2), non-plastidial localization (Ref 3)	Data
At4g22550	LPP β		Prok Gal/Sulpho I Euk Gal/Sulpho Signaling		Seq. Similarity	1 Ref Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.		Data
At5g03080	LPP γ	lpp γ	Prok Gal/Sulpho I Euk Gal/Sulpho Sphingolipid I Signaling	Plastid	Mutant Char.; Heterol Exp'n.; Seq. Similarity	2 Refs Tsegaye, Y., Richardson, C.G., Bravo, J.E., Mulcahy, B.J., Lynch, D.V., Markham, J.E., Jaworski, J.G., Chen, M., Cahoon, E.B., and Dunn, T.M. (2007). Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J. Biol. Chem. 282 , 28195-28206. Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.	Plastidial localization, single mutant lpp- lethal (Ref1), suggested function:LCB-P phosphatases (Ref2). ,Phosphatidate phosphatase activity reported Nakamura et al (2007).Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism.	Data
At5g42870	PAH2	pah2	TAG Synthesis Euk Phospholipid	Cytosol	Mutant Char.; Heterol Exp'n.; Seq. Similarity; Comple. of yeast mutant	4 Refs Han, G.S., Wu, W.I., and Carman, G.M. (2006). The Saccharomyces cerevisiae lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J. Biol. Chem. 281 , 9210-9218. Nakamura, Y., Koizumi, R., Shui, G., Shimojima, M., Wenk, M.R., Ito, T., and Ohta, H. (2009). Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation. Proc. Natl. Acad. Sci. USA 106 , 20978-20983. Eastmond, P.J., Quettier, A.L., Kroon, J.T.M., Craddock, C., Adams, N., and Slabas, A.R. (2010). Phosphatidic acid phosphohydrolase 1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in Arabidopsis. Plant Cell 22 ,2796-2811. Mietkiewska, E., Siloto, R.M.P., Dewald, J., Shah, S., Brindley, D.N., and Weselake, R.J. (2011). Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1 Delta mutant strain of Saccharomyces cerevisiae. Febs Journal 278 , 764-775.	Seq. Similarity to yeast PAH1; Complements yeast PAP mutant; mutant characterization;pah1 pah2 double mutant has decreased soluble PAP activity and during phosphate starvation phospholipid remodelling to DGDG i decreased	Data
At5g66450	LPP ε2	lppε2	Prok Gal/Sulpho I Euk Gal/Sulpho Signaling	Plastid	Mutant Char.; Heterol Exp'n.	1 Ref Nakamura, Y., Tsuchiya, M., and Ohta, H. (2007). Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin. J. Biol. Chem. 282 , 29013-29021.	Plastidial localization, double mutant lpp-1lpp-2 no change in bulk glycerolipid metabolism	Data

Phosphatidate Phosphatase

Enzyme Pathways

Locus Data & Links (May need to scroll right or left to show columns)