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Phospholipid : Diacylglycerol Acyltransferase
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phospholipid |
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2-lysophospholipid |
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1,2-diacylglycerol |
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triacylglycerol |
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Locus Data & Links (May need to scroll right or left to show columns)
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PDAT2
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Endomembrane
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Seq. Similarity
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3 Refs
Stahl, U., Carlsson, A.S., Lenman, M., Dahlqvist, A., Huang, B.Q., Banas, W., Banas, A., and Stymne, S. (2004). Cloning and functional characterization of a Phospholipid:Diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 135 , 1324-1335.Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H., and Stymne, H. (2000). Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc. Natl. Acad. Sci. USA 97 , 6487-6492.Zhang, M., Fan, J.L., Taylor, D.C., and Ohlrogge, J.B. (2009). DGAT1 and PDAT1 Acyltransferases Have Overlapping Functions in Arabidopsis Triacylglycerol Biosynthesis and Are Essential for Normal Pollen and Seed Development. Plant Cell 21 , 3885-3901.
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RT-PCR shows that the gene encoding At3g44830 protein was clearly expressed in developing seeds but only at extremely low levels in leaves and flowers. Since the AtPDAT gene (At5g13640), on the other hand, was constitutively expressed, this indicates that the two proteins might have different physiological functions with At3g44830 perhaps having a specific role in TAG deposition.
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Data
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PDAT1
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Endomembrane
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Mutant Char.; in-planta Overexp'n.; Seq. Similarity
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4 Refs
Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H., and Stymne, H. (2000). Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc. Natl. Acad. Sci. USA 97 , 6487-6492.Stahl, U., Carlsson, A.S., Lenman, M., Dahlqvist, A., Huang, B.Q., Banas, W., Banas, A., and Stymne, S. (2004). Cloning and functional characterization of a Phospholipid:Diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 135 , 1324-1335.Mhaske, V., Beldjilali, K., Ohlrogge, J., and Pollard, M. (2005). Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640). Plant Physiology and Biochemistry 43 ,413-417.Zhang, M., Fan, J.L., Taylor, D.C., and Ohlrogge, J.B. (2009). DGAT1 and PDAT1 Acyltransferases Have Overlapping Functions in Arabidopsis Triacylglycerol Biosynthesis and Are Essential for Normal Pollen and Seed Development. Plant Cell 21 , 3885-3901.
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Seq. Similarity to yeast PDAT; MYB94 activates cuticular wax biosynthetic genes by binding directly to their gene promoter; Loss of function phenotypes not reported
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Data
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