Mutant daisy, kcs2
Gene Name Ketoacyl-CoA Synthase
Gene Abbreviation KCS2 / DAISY
ARALIP Pathway(s) FA Elong & Wax
Suberin I
Evidence For Function Mutant Char.; Heterol Exp'n.; Comple. of yeast mutant
Comments Protein family of 21 members-Root but not seed suberin phenotype. Other family members may have redundant functions. Double mutant of kcs2kcs20 results in 15-20% reduction in cuticular wax. STKCS6 homology to both AtKCS6 and AtKCS5 is similar; it cannot unambiguously be assumed that StKCS6 is the potato orthologue of AtKCS6.
Identification Method
(Lloyd & Meinke, 2012)
Reverse genetics
Mutant Description
(Lloyd & Meinke, 2012)
Short roots
References 1 Lee, S.B., Jung, S.J., Go, Y.S., Kim, H.U., Kim, J.K., Cho, H.J., Park, O.K., and Suh, M.C. (2009). Two Arabidopsis 3-ketoacyl CoA synthase genes, KCS20 and KCS2/DAISY, are functionally redundant in cuticular wax and root suberin biosynthesis, but differentially controlled by osmotic stress. Plant Journal 60 ,462-475.

Fiebig, A., Mayfield, J.A., Miley, N.L., Chau, S., Fischer, R.L., and Preuss, D. (2000). Alterations in CER6, a gene identical to CUT1, differentially affect long-chain lipid content on the surface of pollen and stems. Plant Cell 12 , 2001-€“2008.

Franke, R., Hofer, R., Briesen, I., Emsermann, M., Efremova, N., Yephremov, A., and Schreiber, L. (2009). The DAISY gene from Arabidopsis encodes a fatty acid elongase condensing enzyme involved in the biosynthesis of aliphatic suberin in roots and the chalaza-micropyle region of seeds. Plant J. 57 , 80-€“95.
1
In most cases, the earliest publication for a mutant describes lipid composition data for the mutant. For further publications, please refer to the 'references' column.